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KMID : 1123920030170030751
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Korean Journal of Oriental Physiology and Pathology
2003 Volume.17 No. 3 p.751 ~ p.758
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Effects of Selaginella Tamariscina on Apoptosis via the Activation of Caspase-3 in HL-60
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Nam Hang-Woo
Lee Sung-Won
An Byung-Sang
Chough Won-Joon
Kim Yeong-Mok
Mun Yeun-Ja
Ahn Seong-Hun
Woo Won-Hong
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Abstract
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In our previous studies, we reported that Selaginella Tamariscina(ST) induced apoptotic cell death in HL-60 cells selectively. The cell viability after treatment with extract of ST was quantified by MTT assay and trypan bleu exclusion method. The results showed that application with ST in HL-60 induced 40% cell death at the concentration of 400 ㎍/ml. The cancericidic effect of Selaginella Tamariscina was mediated by apoptosis. Thus, HL-60 cells exposed to Selaginella Tamariscina displayed the DNA fragmentation ladder and nucleus chromatin condensation characteristic for apoptosis. The enzyme activity of caspase-3 and actived caspase-3 protein were markedly increased in HL-60 cells treated with the extract of Selaginella Tamariscina. In addition, the extract of Selaginella Tamariscina induced cleavage of PARP, a known substrate for caspase-3. The expression of Bcl-2, anti-apoptotic protein, was decreased by treatment of the aqueous extract of Selaginella Tamariscina in a dose-dependent manner. And the expression of pro-apoptotic Bax protein was increased. In conclusion, our results suggest that the extract of Selaginella Tamariscina may induce the apoptotic death of HL-60 cells via activation of caspase-3, cleavage of PARP protein, depletion of cellular ATP levels and Bcl-2 degradation.
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KEYWORD
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Selaginella Tamariscina, apoptosis, HL-60, caspase-3, PARP, Bcl-2
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